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recombinant human sdf-1α (cxcl12, 500 μl, 50 μg/ml  (PeproTech)

 
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    PeproTech recombinant human sdf-1α (cxcl12, 500 μl, 50 μg/ml
    Recombinant Human Sdf 1α (Cxcl12, 500 μl, 50 μg/Ml, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human sdf-1α (cxcl12, 500 μl, 50 μg/ml/product/PeproTech
    Average 90 stars, based on 1 article reviews
    recombinant human sdf-1α (cxcl12, 500 μl, 50 μg/ml - by Bioz Stars, 2026-03
    90/100 stars

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    Image Search Results


    GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit-NPs CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); CXCL12: C-X-C motif chemokine 12.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit-NPs CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); CXCL12: C-X-C motif chemokine 12.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques:

    (a) Schematic representation of the perfusion bioreactor system for GBM cells migration assay. (b) The perfusion bioreactor assembly. GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); PDMS: Polydimethylsiloxane; 3D: three-dimensional.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Schematic representation of the perfusion bioreactor system for GBM cells migration assay. (b) The perfusion bioreactor assembly. GBM: Glioblastoma multiforme; Alg 1%: Alginate hydrogel 1%; Alg/Chit NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); PDMS: Polydimethylsiloxane; 3D: three-dimensional.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration

    (a) Hydrogels scans of mCherry-F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 (0.33 μg) under a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 72h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 15, n = 3). Bars represent the mean ± SD and the squares are zoomed regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Hydrogels scans of mCherry-F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 (0.33 μg) under a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 72h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 15, n = 3). Bars represent the mean ± SD and the squares are zoomed regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration, Staining, MANN-WHITNEY

    (a) Hydrogels scans of mCherry F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 72 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Hydrogels scans of mCherry F98 GBM cells migration at t = 0 h, t = 24 h and t = 72 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 72 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was performed using a Mann-Whitney t-test, * p ≤ 0.05, ** p ≤ 0.01. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration, Staining, MANN-WHITNEY

    (a) Hydrogels scans of U87-GFP GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) *, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) *, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Hydrogels scans of U87-GFP GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 0.5 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison post-hoc test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) *, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) *, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration, Staining, Comparison

    (a) Hydrogels scans of GFP U87 GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) **, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Journal: PLOS ONE

    Article Title: CXCL12 impact on glioblastoma cells behaviors under dynamic culture conditions: Insights for developing new therapeutic approaches

    doi: 10.1371/journal.pone.0315038

    Figure Lengend Snippet: (a) Hydrogels scans of GFP U87 GBM cells migration at t = 0 h, t = 24 h and t = 120 h with and without CXCL12 at a flow rate of 3 μL/min. (b) Hoechst staining of the hydrogels at t = 120 h. (c) DNA quantification of the different hydrogel sections at the end of the experiment (N = 9, n = 3). Bars represent the mean ± SD and the squares are zoom regions. Statistical analysis was conducted using a Kruskal-Wallis test followed by Dunn’s multiple comparison test, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. 1 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) **, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) ns. 2 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) **, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 3 : (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12) ns, (Alg:M NPs-Empty vs. Alg:M NPs-CXCL12+) ***, (Alg:M NPs-CXCL12 vs. Alg:M NPs-CXCL12+) ns. 4 : (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12) ns, (Alg:M + cells NPs-Empty vs. Alg:M + cells NPs-CXCL12+) ns, (Alg:M + cells NPs-CXCL12 vs. Alg:M + cells NPs-CXCL12+) **. The scale bar represents 2000 μm. NPs-Empty: Alginate/Chitosan-Nanoparticles unloaded; NPs-CXCL12: Alginate/Chitosan-Nanoparticles loaded with CXCL12; Alg:M: Alginate:Matrigel (50:50); Alg 1%: Alginate 1% hydrogel.

    Article Snippet: Human recombinant CXCL12 (SDF-1α) (purity ≥98%, PeproTech United States, Rocky Hill, NJ, United States) was reconstituted in sterile Milli-Q water following the manufacturer’s instruction.

    Techniques: Migration, Staining, Comparison

    Pathway analysis of the hits identified from the kinome and GPCR/GPCR‐associated RNAi library screens.

    Journal: Immunology and Cell Biology

    Article Title: RNAi library screening reveals Gβ1, Casein Kinase 2 and ICAP‐1 as novel regulators of LFA‐1‐mediated T cell polarity and migration

    doi: 10.1111/imcb.12838

    Figure Lengend Snippet: Pathway analysis of the hits identified from the kinome and GPCR/GPCR‐associated RNAi library screens.

    Article Snippet: Recombinant human SDF‐1α (CXCL12) and IL‐2 were from Peprotech (London, UK).

    Techniques: Activation Assay, Virus, Infection